Colloque

Biosynthesis of β-lactam antibiotics by Streptomyces clavuligerus

Susan Jensen

Membre a labase

Susan Jensen

Résumé du colloque

Streptomyces clavuligerus is a filamentous bacterium which produces β-lactam compounds including penicillins, cephamycins and clavulanic acid. The biosynthetic pathway leading to penicillin/cephamycin compounds has been elucidated and many of the enzymes have been purified. The genes encoding several of these biosynthetic enzymes have been cloned and sequenced. We have concentrated on the enzyme isopenicillin N synthase (IPNS), and have cloned and sequenced the corresponding gene. Transcriptional studies have localized the IPNS promoter and demonstrated that gene expression does not occur from this promoter in either Escherichia coli or in heterologous Streptomyces sp. The enzyme immediately preceding IPNS in the biosynthetic pathway is ACVSynthetase (ACVS). The gene encoding ACVS lies immediately upstream from IPNS and is oriented in the same direction, but is not part of an operon. Purified ACVS protein consists of a single large polypeptide and a second small protein which co-purifies with ACVS. The gene encoding this small ACVS related protein (ACVSR) has been located about 5 kb downstream from IPNS. The gene has been cloned and sequenced, but as yet the function of ACVSR has not been elucidated.

Contexte

Section :

Biologie des actinomycètes

Thème du colloque :

Biologie des actinomycètes

Hôte : Université de Sherbrooke

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Thème du colloque :

Biologie des actinomycètes

Biosynthesis of β-lactam antibiotics by Streptomyces clavuligerus

Susan Jensen