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Certain physico-chemical properties of DNA in its in vivo synthesis in Tribolium confusum Duval

K.D. Chaudhary
A. Lemonde
M.R.V. Murthy
KC

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K.D. Chaudhary

Résumé du colloque

T. confusum DNA has been isolated by the method of Colter et al (1961) which involves repeated extraction of the cells, suspended in buffered 1 M NaCl, with water saturated phenol. The hydrolysate of DNA, obtained by heating the latter in 1N HCl for one hour, was chromatographed on thin-layer DEAE - cellulose (Randerath, 1963) in a solvent system comprising of isobutyric acid, H2O and NH4OH (66:33:1). The ultraviolet spots, corresponding to various bases, were quantitatively estimated and AT/GC ratio was found to approximate a value of 1.4. Certain other properties such as Tm and viscosity measure of T. confusum DNA are under progress. By administering 14C-thymidine in the diet, in vivo synthesis of DNA in this insect has been investigated in relation to growth and metamorphosis. A relatively faster rate of turn-over of DNA occurs when the insect is in an active growth phase.

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Section :
Biochimie
news icon Thème du colloque :
Biochimie
manager icon Responsables :
André Lemonde Roger Daoust
host icon Hôte : Université de Montréal

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