Colloque

Cloning of the PABA synthetase gene of Streptomyces lividans

Francis Arhin

Leo Vining

Membre a labase

Francis Arhin

Résumé du colloque

The PABA synthetase gene of S. lividans was obtained by shotgun cloning of S. lividans 66 genomic DNA into E. coli AB3295 (pabB) using pBR322 as a vector. A 4.8-kb BamHI fragment that complemented the pabA and pabB mutations in E. coli was obtained. Complementation of both mutations was also observed when the same fragment was cloned in the opposite orientation with respect to the vector. The gene was localized on a 7.2-kb BamHI : SstI segment by subcloning into pTZ18R. The 7.2-kb fragment complemented the pab mutation in S. lividans J610 when it was introduced on a Streptomyces - E. coli shuttle vector. However, no plasmid DNA was extractable from pab+ transformants of S. lividans J610. Plasmid DNA was extractable from transformed E. coli AB3295 and Streptomyces griseofuscus C581. When the 4.8-kb BamHI fragment was used to probe genomic digests, hybridization was observed at high stringency with S. lividans 66 and S. lividans M252 but not with other Streptomyces spp., or with L. lactis subsp. lactis. At moderate stringency, hybridization was observed with all Streptomyces spp. tested.

Contexte

Section :

Biologie des actinomycètes

Thème du colloque :

Biologie des actinomycètes

Hôte : Université de Sherbrooke

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Thème du colloque :

Biologie des actinomycètes

Cloning of the PABA synthetase gene of Streptomyces lividans

Francis Arhin