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DNA fingerprinting of Bacillus species based on polymorphism of DNA encoding rRNA

Kwang Bo Joung
Jean-Charles Côté
Young Sup Chung
KB

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Kwang Bo Joung

Résumé du colloque

The genus Bacillus encompasses the Gram-positive sporeforming rod-shaped bacteria. This genus is used for the synthesis of a very wide range of important medical, agricultural, pharmaceutical and other industrial products. This includes a variety of antibiotics, enzymes, amino-acids, and sugars. Some of the Bacillus species play an important role in the biological control of insect pests. The genus Bacillus is phenotypically heterogeneous with members exhibiting an extremely wide range of nutritional requirements, growth conditions, metabolic diversity and DNA base compositions as reported in Bergey's Manual of Systematic Bacteriology. There are increasing evidences of phylogenetic heterogeneity within the genus. Till now, several approaches have been proposed in order to distinguish and classify Bacillus species. Because they have been used on a limited number of Bacillus species and were based on phenotypic data, their ability to discriminate between all species is uncertain. Recently, studies on a variety of microorganisms have demonstrated that rRNA gene restriction pattern analyses can provide a mean of distinguishing between strains. Because this technique is based on DNA-DNA hybridization experiments, it can give more stable, reliable and robust results than any other previous experiments based on phenotypic data. In this report we tested all of the 53 known Bacillus type strains (except B. anthracis), using molecular typing system based on polymorphism of DNA encoding 16S rRNA and 23S rRNA. Moreover, we tested some related genus and E. coli to root the phylogenetic relationship comparisons.

Contexte

Section :
Biologie cellulaire et moléculaire
news icon Thème du colloque :
Biologie cellulaire et moléculaire
host icon Hôte : Université McGill

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