Colloque

Effect of various inhibitors and cofactors on DNA polymerase from Noenetal Rat tissues

M.R.V. Murthy

A.D. Bharucha

Membre a labase

M.R.V. Murthy

Résumé du colloque

DNA polymerase was extracted from nuclei of brain and liver of newborn rats. The effect of various cations, anions, basic and acidic proteins, synthetic and natural polynucleotides, proteolytic enzymes and nucleases were studied in an attempt to understand the nature and kinetics of DNA polymerase activity. Enzyme isolated from brain and liver showed different qualitative and quantitative responses to many of these additives. Protamine was inhibitory at lower concentrations than histone. An enzyme digest of DNA enhanced the synthesis of DNA, even when added to an otherwise full complement reaction mixture indicating a response to oligodeoxynucleotides. The possible significance of these observations in terms of the development of these tissues will be discussed.

Contexte

Section :

Biochimie

Thème du colloque :

Biochimie

Responsables :

Édouard Pagé

Hôte : Université Laval

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