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Exploiting higher harmonics of light...SHG and THG for mapping collagen structure in tissue and malaria infection in blood cells

Paul Wiseman
Santiago Costantino
Jonathan Belisle
PW

Membre a labase

Paul Wiseman

Résumé de la communication

Second harmonic generation (SHG) and third harmonic generation (THG) are relatively new modalities for nonlinear microscopy imaging in tissue. In this presentation, we will describe a home-built multimodal nonlinear laser-scanning microscope that is capable of optical section imaging into tissue using multiphoton excited fluorescence in combination with SHG or THG detection. We will describe use of this microscope for imaging of collagen and elastin distributions in human heart pericardium including diseased tissue. As well we will show how THG provides new windows into imaging of malaria infection. We will describe a new and highly sensitive optical based detection of malaria infected blood cells by THG imaging of the hemozoin pigment that is naturally deposited by the parasite during its lifecycle. The THG signal from the hemozoin was greater than we have observed in any cell type with signal to noise ratios that reach 1000:1 and was detected within blood cells with parasites at the earliest stage of their life-cycle development.

Résumé du colloque

La qualité de la recherche effectuée par les professeurs invités dans le cadre de ce colloque est de haut niveau et permettra de favoriser les échanges entre les différents groupes de recherche en biophotonique au Québec.

Contexte

Section :
Biophotonique : développements et applications
news icon Thème du colloque :
Biophotonique : développements et applications
manager icon Responsables :
François Légaré
host icon Hôte : Institut national de la recherche scientifique

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