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Immunoactivating potential of multilamellar liposome vesicles (MLV) related to the lipid composition

Richard Desjardins
Michel Fournier
Krzysztof Krzystyniak
H.M. Thérien
P. Tancrède
W. Banska
RD

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Richard Desjardins

Résumé du colloque

Non-drug containing multilamellar liposome vesicles (MLV) and small unilamellar vesicles (SUV) of different lipid composition were examined in popliteal lymph node (PLN) enlargement test. Early activation of lymphocyte subsets, induced by MLV and SUV liposomes injected locally into CDI mouse foot pad, was monitored by flow cytometry. Fluorescent monoclonal Ab: anti-L3T4 and Ly2 were used for staining of T cell subsets, anti-Early Activation Marker (EAM), anti-IL-2 Receptor (CD25+) and fluorescent peanut agglutinin (PNA) were used for detection of activated cells. Polyclonal anti-Ig antibody was used for B cell staining. Specific fluorescence patterns observed for ungated lymphocyte subsets and for gated large/activated cells. The data showed significant PLN reaction and increased proportion of activated cells after injection of MLV but not SUV liposomes containing distearoyl phosphatidylcholine (DSPC) and dipalmitoyl phosphatidylcholine (DPPC), characterized by relatively high temperature of transition. Injection of MLV liposomes containing lipids of low transition temperature, such as egg phosphatidylcholine (egg PC) and dimyristoyl phosphatidylcholine (DMPC), did not result in marked PLN reaction. Our data demonstrated a correlation between immunoactivating potential of MLV liposome and its lipid composition.

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Section :
Pharmacologie
news icon Thème du colloque :
Pharmacologie
host icon Hôte : Université du Québec à Montréal

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