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Préparation des monocouches par une méthode nouvelle de mince film en flux laminaire

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Gilles Picard

Résumé du colloque

This paper describes a new way to make protein monlayers rapidly. This process reduces the protein contact time at the air-water interface which subsequently prevents structural alteration. Hight speed protein monolayer preparations are obtained by confining proteins in a thin laminar flow liquid film at various speeds. A prototype made of a rotating glass cylinder compressing cytochrome P450scc validated this method. The cytochrome P450 monolayers were verified by optical microscopy, TEM, STM and AFM. Possible protein 2D crystallites were observed. A rate of protein monolayer preparation up to 2 cm/s was easily obtained. This method is suitable for continuous protein monolayer production and fine particle coating.

Contexte

Section :
Biophysique
news icon Thème du colloque :
Biophysique
host icon Hôte : Université de Trois-Rivières

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