Colloque

Understanding dynamic maturation of different RNPs

Marlene Oeffinger

Membre a labase

Marlene Oeffinger

Résumé de la communication

In recent years, it has become clear that RNAs play an even more significant role within cells than previously thought; they are not only the conveyer of genetic information, but can themselves be regulators of gene expression. All of these RNA species share the fact that, after transcription, they assemble into ribonucleoprotein (RNP) complexes to get modified, processed and transported to their final destination within the cell. However, for each of these RNA species, this maturation occurs by a different pathway defined by very specific processing factors. These factors form discrete subsets of proteins that associate with each species of RNA in a dynamic fashion to define the order of maturation events. The coordination of processing and assembly events is still not well understood, and raises the question of how different processing and assembly factors determine the correctness of the assembly process of maturing RNPs, and, in the case of mRNA, whether each class of RNA is processed by the same subset of factors. We have recently developed a rapid single-step affinity purification method that allows the isolation of transient assembly intermediates and captures the entire pool of bait-associated proteins RNAs. Using this method and further combining proteomic and RNomic techniques, we have set out to determine differences between the maturation of different types of RNPs in S.cerevisiae with the goal to establish dynamic maps of different RNA maturation pathways.

Résumé du colloque

Non. Toutefois, le président et directeur scientifique de l'IRCM, le Dr Tarik Möröy, sera présent au colloque pour accueillir les participants. Dans son mot d'accueil, il présentera la place qu'occupe à l'IRCM la biologie intégrative des systèmes, un nouvel axe de recherche dont font partie les travaux portant sur l'expression et la fonction du génome.