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Non-drug containing multilamellar liposome vesicles (MLV) and small unilamellar vesicles (SUV) of different lipid composition were examined in popliteal lymph node (PLN) enlargement test. Early activation of lymphocyte subsets, induced by MLV and SUV liposomes injected locally into CDI mouse foot pad, was monitored by flow cytometry. Fluorescent monoclonal Ab: anti-L3T4 and Ly2 were used for staining of T cell subsets, anti-Early Activation Marker (EAM), anti-IL-2 Receptor (CD25+) and fluorescent peanut agglutinin (PNA) were used for detection of activated cells. Polyclonal anti-Ig antibody was used for B cell staining. Specific fluorescence patterns observed for ungated lymphocyte subsets and for gated …