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Colloque
Second harmonic generation (SHG) and third harmonic generation (THG) are relatively new modalities for nonlinear microscopy imaging in tissue. In this presentation, we will describe a home-built multimodal nonlinear laser-scanning microscope that is capable of optical section imaging into tissue using multiphoton excited fluorescence in combination with SHG or THG detection. We will describe use of this microscope for imaging of collagen and elastin distributions in human heart pericardium including diseased tissue. As well we will show how THG provides new windows into imaging of malaria infection. We will describe a new and highly sensitive optical based detection of malaria …
Colloque
Second harmonic generation (SHG) and third harmonic generation (THG) are relatively new modalities for nonlinear microscopy imaging in tissue. In this presentation, we will describe a home-built multimodal nonlinear laser-scanning microscope that is capable of optical section imaging into tissue using multiphoton excited fluorescence in combination with SHG or THG detection. We will describe use of this microscope for imaging of collagen and elastin distributions in human heart pericardium including diseased tissue. As well we will show how THG provides new windows into imaging of malaria infection. We will describe a new and highly sensitive optical based detection of malaria …
Colloque
Les interactions protéíne-protéíne à l’intérieure des cellules jouent un rôle clé dans la signalisation cellulaire. Ainsi, il importe de pourvoir mesurer ces interactions dans les différents compartiments cellulaires. Or, réussir à la faire de manière quantitative à l’intérieur des tissus (in situ) demeure un défi de taille. Nous avons développé une nouvelle technique optique qui permet de quantifier la densité et l’oligomérisation de particules fluorescentes à partir d’images individuelles acquises par microscopie confocale. La méthode consiste à ajuster des distributions super-poissonniennes à des histogrammes d’intensités générés à partir d’images. On obtient ainsi une cartographie des densités des particules fluorescentes et …
