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This paper describes a new way to make protein monlayers rapidly. This process reduces the protein contact time at the air-water interface which subsequently prevents structural alteration. Hight speed protein monolayer preparations are obtained by confining proteins in a thin laminar flow liquid film at various speeds. A prototype made of a rotating glass cylinder compressing cytochrome P450scc validated this method. The cytochrome P450 monolayers were verified by optical microscopy, TEM, STM and AFM. Possible protein 2D crystallites were observed. A rate of protein monolayer preparation up to 2 cm/s was easily obtained. This method is suitable for continuous protein …
