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The maximal yield of chlorophyll a fluorescence (Fm) is usually determined experimentally by the closure of photosystem II (PSII) reaction centres with a saturating pulse of light with a minimal time duration of 30 to 50 milliseconds. This duration of saturating light pulse allows for multiple turnovers of PSII which gives rise to a fluorescence yield significantly higher than that induced by a saturating single turnover flash (less than 50 microseconds). Our picosecond time resolved fluorescence apparatus was modified to allow collection of picosecond time resolved fluorescence decay kinetics over a short time window immediately following a saturating single turnover …
