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The PABA synthetase gene of S. lividans was obtained by shotgun cloning of S. lividans 66 genomic DNA into E. coli AB3295 (pabB) using pBR322 as a vector. A 4.8-kb BamHI fragment that complemented the pabA and pabB mutations in E. coli was obtained. Complementation of both mutations was also observed when the same fragment was cloned in the opposite orientation with respect to the vector. The gene was localized on a 7.2-kb BamHI : SstI segment by subcloning into pTZ18R. The 7.2-kb fragment complemented the pab mutation in S. lividans J610 when it was introduced on a Streptomyces - …
