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The development of biosensors for point-of-care diagnosis requires on- field analysis accompanied by real-time data acquisition. While SPR imaging (SPRi) biosensors could potentially be the best suited candidate for biomarker-based diagnosis, there remains many challenges to overcome. In particular, they are limited by the flow-cell technology to serial-sample processing and have limited sensitivity for the detection of markers present at low concentration. In this work, we explored SPRi technology and implemented an approach to increase sensitivity and enhance surface plasmon resonance imaging detection of DNA binding interactions. We have developed a droplet-based digital microfluidic platform with an integrated nanostructured biosensor …
